STUDY ON IN VITRO REGENERATION SYSTEM AND HAIRY ROOT INDUCTION IN CHINESE ACONITE (ACONITUM CARMICHAELII DEBEAUX) PLANT

Abstract

In this study, Chinese aconite (Aconitum carmichaelii Debeaux), a precious medicinal plant, collected from Quan Ba, Ha Giang province, Vietnam, was investigated for in vitro regeneration and hairy root induction. The optimal medium for multi-shoot induction from stem segments was MS + 30 g L^-1 saccharose + 9 g L^-1 agar + 1.5 mg L^-1 BAP with the result of 4.57 shoots per explants. The maximum roots per shoot after 8 weeks of culture was achieved with MS medium supplemented with 0.5 mg L^-1 IBA at 2.70 ± 0.04 (roots /a shoot). Hairy roots were produced from root explants of A. carmichaelii infected by Agrobacterium rhizogenes strain ATTC 15834 and cultured on MS + 30 g L^-1 saccharose + 500 mg L^-1 cefotaxime + 100 μmol L^-1 AS. The highest fresh weight of hairy roots was obtained in liquid medium under shaking conditions, with 3.22 g of fresh weight. After 6 weeks, the hairy root mass was increased 5.85-fold compared to the initial mass. In vitro regeneration system will be used for gene expression analysis, and hairy root lines will be used to obtain bioactive compounds.