STUDYING ANTIMICROBIAL ACTIVITY OF BACTERIOPHAGES ON MULTI-ANTIBIOTIC RESISTANT ESCHERICHIA COLI ISOLATED FROM TRA FISH (Pangasius hypophthalmus)

Abstract

This study aims to investigate the inactivation ability of bacteriophages derived from the natural environment on pathogenic bacteria isolated from Pangasius farming and processing chain. The inactivation ability of bacteriophages was specically studied on multi-antibiotic resistant Escherichia coli strain at different doses of phage (0-100 μL), initial bacteria counts (2, 3 and 4 log CFU/mL) and incubation temperature (37 and 7 ± 1^oC). The results showed that a variety of the isolated bacteria were infected by bacteriophages from pond water, mud bottom and Pangasius feces, such as: Escherichia coli (12/18); Vibrio cholerae (1/8); Staphylococcus aureus (1/7) and Salmonella spp. (4/9). In addition, the results showed a reduction of  multi-antibiotic resistant E. coli 80ENV strain, depending on phage doses, bacterial quantity, incubation temperature and exposure time. Antimicrobial activity of phages at 7 ± 1^oC was better than 37^oC and the best activity within 2 hours. Overall, the results indicate that the bacteriophages may be useful in the control of food-borne pathogens and antibiotic resistant bacteria.