RESEARCH ON IN VITRO CULTURE OF HOYA MULTIFLORA SPECIES

Abstract

Hoya multiflora is a beautiful ornamental plant decoration. In addition, Hoya multiflora also works very well in treating a number of diseases, which has been scientifically proven. The conservation of medicinal plants in general and Hoya multiflora in particular can be done by many traditional methods such as sowing seeds after harvesting old ripe fruits and choosing leaves or old stalks with roots to planted to the ground. However, the achieved effect is not high, rarely has ripe fruit to get seeds. In this study, we used in vitro culture method to find a culture medium suitable for plant growth and development in vitro. The results showed that the medium supplemented with BAP 1.0 mg/l gave the best multi-shoot efficiency in the in vitro concentrations of Hoya multiflora. In this medium, green shoots, well developed (3.28 shoots/sample). Optimal medium for the elongation of shoots supplemented with GA3 0.5 mg/l (4.84 cm/sample). The complete plant formation is most suitable on basal MS medium supplemented with α-NAA at a concentration of 0.5 mg/l. The number of roots obtained was 4.83 roots/sample after 8 weeks. The survival rate of seedlings when planted on natural substrates reached 88.3%.