IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE

Abstract

The chrysanthemum is one of the most widely cultivated plants and also one of the most important ornamental plants in the world. In this study, we established a systematic regeneration directly adventitous shoots from the leaf segments of chrysanthemum, then determined the concentration of NaCl as a direct selective factor for the adventitous shoots, and finally analyzed the genetic diversity of some selected clones by ISSR-PCR analysis. The results showed that the most suitable medium for the adventitous shoot regeneration from leaf explants in chrysanthemum was MS, 30 g/l sucrose, 7 g/l agar, pH 5.8, supplemented with 0.5 mg/l BAP. After 5 cultured weeks, the number of shoots per explants, the number of leaves per shoot and the length of shoot were 4.37 (shoots/explant), 4.25 (amount of leaves/shoot) and 1.96 (cm), respectively. The medium for direct selection of salinity-tolerant in vitro shoots was the adventitous shoot regeneration medium supplemented with 100 mM NaCl. The 873 primer [5 '- (GACA)₄ -3'] was suitable for analyzing the genetic relationships of selected chrysanthemum lines. By ISSR-PCR analysis, the following five selective chrysanthemum lines (D1-D5) showed the genetic diversity. These chrysanthemum lines are potential materials for more analysis of salinity tolerance.