CHARATERISTICS OF MATK SEQUENCE ISOLATED FROM Nervilia fordii (Hance) Schlechter COLLECTED IN CAO BANG PROVINCE

Abstract

Nervilia fordii (Hance) Schlechter, which belongs to the genus Nervilia in the family Orchidaceae, contains terpenoids, flavonoids, amino acids and some volatile oils. These subtances have anti-inflammatory and anti-viral activities as well as reduce pain, cough, asthma and chronic bronchitis. Using matK sequence analyzing method, matK sequence dimension of LML-02-CB sample was found at 778bp, in which A type nucleotide is 236 (30.33%), C type is 120 (15.42%), G type is 115 (14.78%), T type is 307 (39.46%). As such, matK sequence of LML-02-CB sample is 99.08% similar to that of Nervilia aragona coded as JN004498 on GenBank. matK zone similarity index on nucleotide sequence of LML-02-CB sample compared to 5 matK sequences on GenBank varies between 97.4% to 99.5%. It is possible to use matK indicator as one of molecular indicators in assessing single leaf orchid species.