GENETIC DIVERSITY OF SPINIBARBUS DENTICULATUS POPULATIONS USING MOLECULAR MAKER

Abstract

Three populations of Spinibarbus denticulatus collected in Ha Giang, Tuyen Quang, and Hoa Binh were analyzed for genetic diversity based on COI gene sequence analysis. Results showed that the studied populations have relatively high haplotype diversity (Hd) and moderate nucleotide diversity (π). Eleven haplotypes were detected out of a total of 90 analyzed sequences. All haplotypes sequences were deposited in the GenBank database, with accession numbers from MW446147 to MW446157. In terms of genetic differentiation, F_ST presented that there was a large genetic difference among three populations, in which F_ST values between Ha Giang and Tuyen Quang was the largest (0.80807). Analysis of molecular variance (AMOVA) indicated that three studied fish populations were different among others because most of the genetic variation occurred between populations (64.83%). The populations had a relatively large genetic distance and a clear population structure. Analytical data revealed a lack of migration or gene flow between populations.