ISOLATION AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC BACTERIAL STRAINS IN Pouzolzia zeylanica L.

Abstract

In recent years, many studies have shown that endophytic bacteria in plants are essential in agriculture, medicine and have great potential for applications. Therefore, the aim of this study is isolating endophytic bacteria in Pouzolzia zeylanica L.. Root, stem and leaf samples of P. zeylanica in a local area in Can Tho city were used as raw materials for isolating endophytic bacteria. 26 endophytic bacterial strains were isolated, showing the ability to culture in vitro. The antibacterial activity of those bacteria was investigated by the agar well diffusion method for intracellular, extracellular and the mixture of culture media and bacteria cells. 17 strains showed antibacterial activity against Pseudomonas aeruginosa, Listeria innocua, Vibrio parahaemolyticus and Staphylococcus aureus. The mixture and extracellular fluid have high antibacterial ability, while intracellular fluid does not. This study gives a hint for applying endophytic bacteria as a biocontrol resource.