CHARACTERIZATION OF SILVER NANOPARTICLES SUBSTRATES USING LASER INDUCED REDUCTION TOWARD SURFACE-ENHANCED RAMAN SPECTROSCOPY

Abstract

Surface-enhanced Raman spectroscopy (SERS) has garnered significant attention due to its efficacy in elucidating chemical and structural information from pharmaceutically active compounds (PhACs). The advancement and practical utilization of SERS technology rely heavily on the fabrication of suitable SERS substrates. Integration of SERS substrates within microfluidic systems introduces additional complexity to this task. This investigation employs a laser-induced reduction technique to fabricate silver nanoparticle substrates directly within microfluidic environments. The study aims to evaluate the influence of irradiation time and laser power on substrate quality. Subsequently, these substrates are employed for the characterization of Rhodamine B. The findings reveal that an irradiation time of 30 seconds and a laser power of 0.11 mW yield optimal results, manifesting nanoparticle sizes within the range of 20-40 nm with a well-organized arrangement. Notably, the system exhibits a sevenfold increase in Raman signal compared to non-SERS conditions, thereby validating its efficacy.