Detection of alpha-amanitin encoding gene utilizing real-time PCR technique

Abstract

Given the limited taxonomy of toxic fungi as well as the large number of mushroom species, identification of mycotoxins in mushroom samples can accelerate information gathering and progress, while increasing Enhanced testing accuracy of testing. In that situation, it is necessary to research methods to identify toxin-carrying genes in mushrooms, to help monitor and classify poisonous mushrooms, disseminate and increase people's awareness of food safety. done faster and more accurately. The research team succeeded in developing a real-time PCR method to detect the toxin alpha-amanitin gene on mushrooms with a detection limit of 101 copies/µL. The study has fully verified the correct parameters, 100% accuracy, and specificity.