CONSTRUCT CALIBRATION CURVE FOR DETERMINING ACETONITRILE BY GAS CHROMATOGRAPH

Abstract

18F-FDG radiopharmaceuticals used for diagnostic imaging by PET, PET/CT.
According to the standards of the world's pharmacopoeias such as British
Pharmacopoeia (BP), US (USP), Europe (Ph.Eur), the amount of residual solvent -
Acetonitrile (ACN) in 18F-FDG radiopharmaceuticals does not exceed 0.41mg/mL.
To ensure high accuracy analysis results, gas chromatography need to pass the
repeatability test before proceeding construct calibration curve and measure.
The Agilent GC7890B gas chromatograph, which contains flame ionization
detector (FID), HP-INNOWax column (19091N-133), autosampler was used in
this research; determine the repeatability (n = 10) of the same ACN solution
showed that the RSD of retention time is 0.0345%; RSD of peak height is
1.3784%; RSD of peak width is 1.283% and RSD of peak area is 1.1376% (RSD <
3.7% the repeatability is qualified); calibration curve from 0.1; 0.2; 0.3; 0.4; 0.5;
0.6 (mg/mL) concentrations has the linear regression equation y = 433.280238.x
- 12.431278 and the correlation coefficient R = 0.99813.