Selection of native Bacillus strains with the ability plant growth promoting and controlling root-knot nematode Meloidogyne spp.

Authors

  • Thi Van Tran
  • Thi Hong Dieu Nguyen
  • Nguyen Thi Van Khanh
  • Minh Quang Nguyen
  • Truong Phuoc Thien Hoang
  • Thi Thu Ha Tran
  • Uyen Tran Da Dao
  • Thi Thuy Hue Vo
  • Le Dinh Don

Keywords

Bacillus spp., Meloidogyne spp., Tuyến trùng bướu rễ

Abstract

The study aimed to identify beneficial indigenous microorganisms to control root-knot nematodes on vegetables. This research isolated, selected, and identified three promising indigenous Bacillus strains (Bacillus velezensis BHMT 4.1, B. amyloliquefaciens CCCX 2.2, and B. subtilis CCCX 2.1) for controlling root-knot nematodes (Meloidogyne spp.) and promoting vegetable plant growth. The study qualitatively assessed several plant growth-promoting traits of Bacillus strains. All of them were capable of producing IAA (indole-3-acetic acid), solubilizing phosphate (with solubilization halos ranging from 1.57 to 2.0 mm), and producing siderophores (with siderophore production indices from 3.33 to 6.33 mm). Furthermore, three Bacillus strains exhibited nematicidal activity against the second juvenile stage (J2) and inhibited egg hatching of Meloidogyne spp. B. velezensis BHMT 4.1 strain demonstrated the highest mortality rate of 66.67% against J2 after 48 h and achieved a 44% inhibition rate of egg hatching after 7 days. These findings suggest that indigenous Bacillus strains have strong potential for application in the biological control of root-knot nematodes (Meloidogyne spp.) on vegetables.

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Published

2026-04-25

Issue

Vol. 25 No. 2 (2026)

Section

Công nghệ sinh học