Analysis of genetic diversity of Cymbidium finlaysonianum based on SSR markers
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Abstract
Cymbidium finlaysonianum is one of the Vietnamese orchid species, diverse in shape and color of flowers, with high economic value, but the genetic resources of this species have been depleted due to overexploitation. A total of 32 C. finlaysonianum samples were collected and evaluated for genetic diversity based on SSR markers. The results showed that 12 primers showed high polymorphism with an average polymorphic allele frequency of 0.22 and an average allele number of 3.5 alleles/primer, ranging from 2 to 6 alleles/primer. In addition, the high genetic diversity between these samples was also recorded through the polymorphic information content of 0.79, ranging from 0.58 to 0.94. The results of genetic relationship analysis showed that these samples had genetic diversity coefficients ranging from 0.02 to 0.4 with an average of 0,17. Cluster analysis displayed that 32 C. finlaysonianum samples were divided into 06 main groups. Group (Ia, Ib) consisted of 18 samples, group Ic comprised 04 samples, group IIa comprised 07 samples, group IIb comprised 02 samples, and group III showed only 01 sample. These results could be useful information for the genetic conservation and development of new C. finlaysonianum varieties in the future.
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References
Anuttato, S., Boonruangrod, R., Kongsamai, B., & Chanprame, S. (2017). Morphological characterization of wild Rhynchostylis gigantea in Thailand. Journal of International Society for Southeast Asian Agricultural Sciences 23(2), 20-32.
Chandra, D. L., Pathak, P., Rao, A. N., & Rajeevan, P. K. (Eds.). (2015). Commercial orchids. Berlin, Germany: De Gruyter Open.
Chung, Y. L., Kuo, Y. T., & Wu, W. L. (2017). Development of SSR markers in Phalaenopsis orchids, their characterization, crosstransferability and application for identification. Singapore: World Scientific.
Huang, J. L., Zeng, C. X., Li, H. T., & Yang, J. B. (2011). Isolation and characterization of 15 microsatellite markers from the spring orchid (Cymbidium goeringii) (Orchidaceae). American Journal of Botany 98(4), e76-e77. http//doi. org/10.3732/ajb.1000446.
Hyun, Y. S., Kim, J., & Chung, K. W. (2012). Development of polymorphic microsatellite markers for Cymbidium goeringii (Orchidaceae). American Journal of Botany 99(5), e193-e198. http//doi.org/10.3732/ajb.1100505.
Lee, H. J., Park, H. R., Lee, A. J., Nam, D. E., Lee, D. G., Do, Y., & Chung, K. W. (2020). Genetic authentication of cultivars with flowervariant types using SSR markers in spring orchid, Cymbidium goeringii. Horticulture, Environment, and Biotechnology 61(3), 577-590.
Li, X., Jin, F., Jin, L., Jackson, A., Huang, C., Li, K., & Shu, X. (2014). Development of Cymbidium ensifolium genic SSR markers and their utility in genetic diversity and population structure analysis in cymbidiums. BMC Genetics 15, 124. http//doi.org/10.1186/s12863-014-0124-5.
Moe, K. T., Zhao, W., Song, H. S., Kim, Y. H., Chung, J. W., Cho, Y. I., Park, P. H., Park, H. S., Chae, S. C., & Park, Y. J. (2010). Development of SSR markers to study diversity in the genus Cymbidium. Biochemical Systematics and Ecology 38(4), 585-594.
Nayak, N. R., Tanaka, M., Teixeira, D., & Silva, J. (Eds.). (2006). Biotechnology of Cymbidium - An overview of recent progress and future opportunities. Isleworth, UK: Global Science Books.
Silva, T. J. A., Jin, X., Dobránszki, J., Lu, J., Wang, H., Zotz, G., Cardoso, J. C., & Zeng, S. (2016). Advances in Dendrobium molecular research: Applications in genetic variation, identification and breeding. Molecular Phylogenetics and Evolution 95, 196-216. http//doi.org/10.1016/j.ympev.2015.10.012.
Tran, H. (Ed.). (2000). The orchids of Vietnam. Ho Chi Minh City, Vietnam: Agricultural Publishing House.
Xia, K., Ye, X., & Zhang, M. (2008). Isolation and characterization of nine microsatellite markers for Cymbidium sinense. HortScience 43(6), 1925-1926. https://doi.org/10.21273/HORTSCI.43.6.1925.
Yang, J. B., Tang, M., Li, H. T., Zhang, Z. R., & Li, D. Z. (2013). Complete chloroplast genome of the genus Cymbidium: lights into the species identification, phylogenetic implications and population genetic analyses. BMC Evolutionary Biology 13, 84. https://doi.org/10.1186/1471-2148-13-84.
Zhao, T. M., Zheng, S. G., Hu, Y. D., Zhao, R. X., Li, H. J., Zhang, X. Q., & Chun, Z. (2019). Classification of interspecific and intraspecific species by genome-wide SSR markers on Dendrobium. South African Journal of Botany 127, 136-146. https://doi.org/10.1016/j.sajb.2019.08.051.
